SRajamaniTAGC 2020 poster.pdf (2.16 MB)
Tagging Juvenile Hormone receptors in Drosophila
poster
posted on 2020-04-20, 23:52 authored by Saathvika Rajamani, LiYing Wei, Edward DubrovskyJuvenile Hormone (JH) is a key component of the insect endocrine system
that regulates many functions. In Drosophila, JH elicits its action through the
paralogous bHLH receptors, Methoprene tolerant (Met) and Germ
cell-expressed (Gce). An important aspect of receptor functioning lies in its
subcellular localization, and it has been identified that receptor localization is
regulated by both hormone dependent and independent mechanisms. To
better understand localization, we generated two epitope tagged receptor fly
lines using CRISPR/Cas9 technology. The receptors were tagged at their
carboxy termini with different epitopes, Met with V5 and Gce with 3xFLAG.
The generated models were confirmed for the expression of epitope tagged
receptors through western blot, followed by immunostaining for studying the
subcellular localization in various tissues at different developmental stages.
We observed localization to be predominantly nuclear. These models will be
used to study localization of receptors in a JH-deficient background to study
hormone dependent localization of the receptors.