Regulation of Protein Kinase A (PKA) activity to mediate chromosome segregation in Saccharomyces cerevisiae
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As a first step toward determining the mechanism of PKA regulation we measured the expression and activity of PKA at different cell cycle stages. Our initial results show that PKA activity increases through the cell cycle although protein levels remain constant. Associated with the increase in PKA activity we also observe increasing levels of Dam1 phosphorylated at the PKA site.