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Method to Culture and Assay Large-Scale Mixed-Stage C.elegans populations for Omics Studies

posted on 20.04.2020, 22:46 by Amanda O. Shaver, Arthur S. Edison, Goncalo Gouveia, Erik C. Andersen, Pamela S. Kirby
Presentation of poster 608B at TAGC 2020 Online. File includes a 10-minute video walkthrough of the poster material (Shaver_TAGC2020_Poster608B_PosterWalkThrough.mov).

Here, a method to culture and collect an abundant mixed-stage C. elegans population on a large-scale culture plate (LSCP) and subsequent phenotypic data is presented. This pipeline yields sufficient numbers of animals to collect phenotypic and population data, along with any data needed for omics experiments (i.e. genomics, transcriptomics, proteomics, and metabolomics). In addition, the LSCP method requires minimal manipulation to the animals themselves, less user prep time, provides tight environmental control, and ensures that handling of each sample is consistent throughout the study for overall reproducibility. Lastly, methods to document population size, population distribution, and presence/absence of C. elegans life stages in a given LSCP are presented. This work is currently under review with JoVE.

My online poster presentation time is Monday, April 27, 2020 from. 1:36 - 1:38 PM.

NOTE: Name of poster on abstract is "Utilizing the Diversity of C. elegans to Identify Unknown Metabolites", however poster title was changed to better reflect the content of the poster.

Any questions about the poster can be sent to amanda.shaver@uga.edu or you can find me at Twitter @aoshaver.




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