Investigating the Quantitative Relationship Between Transcription Factor and Target in Yeast

We propose a gene regulation function that quantitatively relates transcription factor abundance to target gene expression level. This quantitative relationship is effectively unknown, and we aim to fill this knowledge gap by titrating Saccharomyces cerevisiae transcription factors and quantifying genome-wide gene expression response in their targets using RNA sequencing. We can use this function to uncover the structure of the gene regulatory network in yeast by titrating the expression level of the transcription factor RAP1.