TAGC 2020 Poster_Pandey Ashutosh.pdf (11.89 MB)

Glycosylation of specific Notch EGF repeats by Ofut1 and Fringe regulates Notch signaling in Drosophila

Download (11.89 MB)
poster
posted on 20.04.2020, 23:51 by Ashutosh Pandey, Beth M. Harvey, Mario F Lopez, Atsuko Ito, Robert S. Haltiwanger, Hamed Jafar-Nejad
We have reported important roles in Drosophila Notch signaling for GlcNAc-fucose-O glycans on three sites: EGF8, 9 and 12. O-fucose monosaccharide on EGF12 (in the absence of Fringe) is essential for Delta-mediated lateral inhibition in embryos. However, wing vein development depends on addition of GlcNAc to EGF8 and 12 by Fringe, with minor contribution from EGF9. Fringe modifications of EGF8 and 12 together prevent Notch from cis-inhibiting Serrate, thereby promoting normal wing margin formation. Our work shows the combinatorial and context-dependent roles of GlcNAc-fucose-O glycans on these sites in Drosophila Notch-ligand interactions.

Funding

Regulation of developmental signaling pathways by glycosylation and deglycosylation

National Institute of General Medical Sciences

Find out more...

Fine-Tuning the Notch Signaling Pathway via O-Glucosylation

National Institute of General Medical Sciences

Find out more...

O-Glycosylation of Epidermal Growth Factor-like Motifs

National Institute of General Medical Sciences

Find out more...

History

Program Number

1621A

Licence

Exports

Logo branding

Licence

Exports