G body nucleation, mobility and localization, and degradation in Saccharomyces cerevisiae

G body dynamics and localization was explored in our study. By conducting glk1 knockout experiments we determined that Glk1 is required for G body formation. We also determined that G bodies are degraded overtime. These data motivated our study of G body intracellular localization experiments using GFP tagged Pfk2 and organelle stains. We determined that G bodies localize to vacuoles more often than mitochondria, and that G bodies are not static and remain closely associated with vacuoles over long periods of time.