Functional analysis of RPN-13, a C. elegans proteasome subunit, using a strain that indicates proteolytic function in the germline
posterposted on 20.04.2020 by Caroline Ugoaru, Lourds Michelle Fernando, Anna Anna
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This data shows the functional analysis of RPN-13, a C. elegans 26S proteasome subunit, using a strain that indicates proteolytic function in the germline. RNAi was used to analyze the effects of suppressing rpn-13 and codepleting rpn-13 and wee-1.3, on C.elegans fertility. Strain IT1877 contains a mutated ubiquitin fused to a GFP, and therefore monitors proteolytic activity of the 26S Proteasome. If gene depletions cause 26S proteasome dysfunction, the degree of dysfunction can be quantified by measuring the intensity of the emitted signal. A brighter GFP signal indicates proteasome dysfunction, while low to minimal GFP signal means the proteasome is functioning normally. This strain was used to study the effects of rpn-13 suppression on 26S proteasome activity.