Bone_Rosikon_2279B.pdf (18.74 MB)

Embryonic asymmetry and development of functional neuronal connectivity

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posted on 20.04.2020 by Megan Bone, Katarzyna Rosikon
Embryonic cell division was observed in both Wild type (N2) and del.gpa-16 C. elegans. N2 worms presented 100% normal embryonic cell division. While the absence of del.gpa-16 , resulted in 50% normal embryonic cell division, over 30% divided abnormally. All N2 adult worms were dextral and 30% of the del.gpa-16 mutants had reversed gut and gonad placement. Both strains of del.gpa-16 were abnormal in non-associative learning specifically, habituation. Compared to N2, del.gpa-16 required more touches to habituate (40). del.gpa-16 mutants are defective in associative learning. Isoamyl alcohol was used to condition the worms in the absence of food. When transferred to test plates, N2 worms avoided the isoamyl alcohol while del.gpa-16 mutants maintained their attraction (n=7). Connectivity of sensory neuron ASEr with interneuron AIY was observed. Wild type animals show stereotypic ASEr location while some of the del.gpa-16 animals showed reversed ASEr neuron location.

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NIGMS-1P20GM103653-01A1; NSF EiR 1900212

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2279B

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