DNA methylation is required for expression of regional gene expression programs in the zebrafish intestine
posterposted on 20.04.2020 by Gilberto Padilla Mercado, Colin R. Lickwar, Jia Wen, Lindsey Marjoram, Michel Bagnat, John F. Rawls
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Describes findings that DNA methylation is important for small intestinal signature gene expression programs. Uses zebrafish transgenics (4.5fabp2:DsRed, NFkB:GFP, cldn15BAC:GFP, tnfa:GFP). Includes exploratory data analysis of RNA-Seq and single-cell RNA seq data, the latter analyzed with the package Seurat. Live stereoscopic fluorescence imaging quantified with FIJI image processing software.