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An efficient, tunable solution for pre-capture multiplexing in targeted sequencing via novel chemistry and semiconductor DNA synthesis technology
poster
posted on 2020-04-20, 22:42 authored by Jiashi Wang, Cheng Song, Raymond Miller, Jianpeng Wang, Lumeng Ye, Hong Li, Heidi HuangPre-capture
multiplexing is a cost-effective and efficient way for targeted next-generation
sequencing. To allow high-throughput pre-capture pooling without compromising
the sequencing quality, we developed a series of reagents, including 384+ plex
customizable adaptors, TRUE universal blockers, and a fast and cost-effective
hybrid capture solution using semiconductor DNA synthesis technology. Our data
shows our adaptors have an extremely lost cross-contamination rate, which can
minimize the index hopping during pre-capture pooling. The TRUE universal
blockers using unique chemistry can bind to adapters regardless of the index
length, simplify the workflow and still yield consistent capture efficiency
during sample multiplexing. Also, the GenFisher capture probes, produced by
semiconductor DNA synthesis technology, can deliver high capture efficiency
after pre-capture pooling, with the potential of evolution via the test-learn
cycle.