Temperature induced DNA damage is associated with increased transposon mobility in spermatocyte nuclei
Nicole Kurhanewicz
Devin Dinwiddie
Zachary Bush
Diana E. Libuda
10.6084/m9.figshare.12151353.v1
https://tagc2020.figshare.com/articles/poster/Temperature_induced_DNA_damage_is_associated_with_increased_transposon_mobility_in_spermatocyte_nuclei/12151353
<p></p><p>Sexually-reproducing organisms use meiosis to generate
haploid gametes, such as sperm and eggs, to transmit their genome to the next
generation. All tissues are susceptible
to dramatic increases in temperature; however, developing sperm in the testes
are unusually sensitive to small temperature fluctuations. Failure to
thermoregulate spermatogenetic tissue and prolonged exposure to elevated
temperatures are linked to male infertility.
Further, temperature increases are known to cause DNA damage in
spermatocytes, but the molecular mechanisms underlying this damage are
unclear. Here we show that upon a brief
heat-shock, the spermatocytes (but not oocytes) of <i>Caenorhabditis elegans</i>
exhibit an increase in double strand DNA breaks (DSBs), that these temperature-induced DSBs occur via
a SPO-11 independent pathway, and occur concurrent with impaired male fertility
and temperature-induced transposon activity within the genome.</p>
<p> </p><br><p></p>
2020-04-20 20:47:10
DNA repair events
transposable element content
heat exposure
Cell Biology
Genome Structure and Regulation
Genetics
Molecular Biology