Microtubules regulate intracellular trafficking to mediate apical constriction during tissue invagination
Thao Le
SeYeon Chung
10.6084/m9.figshare.12150450.v1
https://tagc2020.figshare.com/articles/poster/Microtubules_regulate_intracellular_trafficking_to_mediate_apical_constriction_during_tissue_invagination/12150450
<p>The
Drosophila embryonic salivary gland (SG) invaginates by budding to form a
three-dimensional tube. Coordinated apical constriction during SG invagination
is critical for proper tube shape. We previously showed that Folded
gastrulation (Fog)-dependent Rho-associated kinase (Rok) accumulation in the apicomedial region of the SG cells is required
for apicomedial myosin formation and clustered
apical constriction near the invagination pit. Here, we show that microtubule
(MT)-dependent intracellular trafficking has a role in regulating apical
constriction during SG invagination. Key components involved in protein
trafficking, including dynein heavy chain, Rab11 and Nuclear fallout (Nuf), are apically enriched near the
invagination pit in a MT-dependent manner during SG invagination. This
enrichment is crucial for apical constriction as disruption of the MT networks
or intracellular trafficking impairs formation of apicomedial myosin, which leads to apical
constriction defects. We show that apical transport of several proteins along
MTs, either in a Rab11-dependent or independent manner, mediates clustered
apical constriction during SG invagination. Key proteins that are transported
include the Fog ligand, the apical determinant protein Crb, the key adherens junction protein E-Cad and the
scaffolding protein Bazooka/Par3, and knockdown of these genes in the SG
results in apical constriction defects. These results define a role of
MT-dependent intracellular trafficking in regulating the actomyosin networks ands
cell junctions to coordinate cell behaviors during tubular organ formation.</p>
2020-04-20 21:54:06
microtubules
intracellular trafficking
apical constriction
junctional proteins
fog signaling
Developmental Biology