10.6084/m9.figshare.12150417.v2
Raghuvir Viswanatha
Raghuvir
Viswanatha
Enzo Mameli
Enzo
Mameli
Jonathan Rodiger
Jonathan
Rodiger
Yanhui Hu
Yanhui
Hu
Naoki Yamanaka
Naoki
Yamanaka
Norbert Perrimon
Norbert
Perrimon
Development of CRISPR knockout screening in mosquito cell lines
TAGC 2020
2020
CRISPR
CRISPR screen
Drosophila
Anopheles
Mosquito
Ecdysone
Molting Hormone
Genetics
Genomics
Invertebrate Biology
2020-04-22 01:41:24
Poster
https://tagc2020.figshare.com/articles/poster/Development_of_CRISPR_knockout_screening_in_mosquito_cell_lines/12150417
Insect cell culture has been a valuable tool to dissect conserved pathways that are simpler in insects than mammals as well as insect-specific biology: interactions with insect hormones, pathogens, toxins, and drugs. Pooled format screens such as CRISPR screens had been unavailable in insect cell-lines due to an inability to conduct massively parallel delivery of large CRISPR guide libraries. In this poster, we share our results on developing CRISPR screening in Drosophila (fruit fly) cells and show a recently published use case. In unpublished work, we have now adapted a cell-line from Anopheles gambiae (malaria mosquito) and validate the approach using pilot gene-by- drug screens. We also describe an online resource for preparing CRISPR libraries for mosquito cell-lines.