Functional analysis of RPN-13, a C. elegans proteasome subunit, using a strain that indicates proteolytic function in the germline
Caroline Ugoaru
Lourds Michelle Fernando
Anna Anna
10.6084/m9.figshare.12149814.v1
https://tagc2020.figshare.com/articles/poster/Functional_analysis_of_RPN-13_a_C_elegans_proteasome_subunit_using_a_strain_that_indicates_proteolytic_function_in_the_germline/12149814
This data shows the functional analysis of RPN-13, a <i>C. elegans</i> 26S proteasome subunit, using a strain that indicates proteolytic function in the germline. RNAi was used to analyze the effects of suppressing <i>rpn-13 </i>and codepleting <i>rpn-13</i> and <i>wee-1.3, </i>on C.elegans fertility<i>.</i> Strain IT1877 contains a mutated ubiquitin fused to a GFP, and therefore monitors proteolytic activity of the 26S Proteasome. If gene depletions cause 26S proteasome dysfunction, the degree of dysfunction can be quantified by measuring the intensity of the emitted signal. A brighter GFP signal indicates proteasome dysfunction, while low to minimal GFP signal means the proteasome is functioning normally. This strain was used to study the effects of <i>rpn-13</i> suppression on 26S proteasome activity.
2020-04-20 22:27:41
elegans
C. elegans germ line
Caenorhabditis elegans
germ cell line
rpn
Animal Cell and Molecular Biology
Developmental Biology
Developmental Genetics (incl. Sex Determination)
Genomics
Molecular Biology
Genetics
Genome Structure and Regulation