10.6084/m9.figshare.12149796.v1 Karen Schmeichel Karen Schmeichel Onur Birol Onur Birol David Katz David Katz Sindy Chavez Sindy Chavez Brandon Carpenter Brandon Carpenter Jovan Brockett Jovan Brockett Characterization of a potential gene interaction between spr-5, met-2, and mep-1 in determining germline versus soma in C. elegans TAGC 2020 2020 Caenorhabditis elegans bioinformatics analyses Epigenetic Modifications Development cell biology experiments RNAi knockdown mutants Differentially expressed gene (DEG) transcriptomics data Bioinformatics Cell Biology Epigenetics (incl. Genome Methylation and Epigenomics) Genetics Gene Expression (incl. Microarray and other genome-wide approaches) Genomics Molecular Biology Quantitative Genetics (incl. Disease and Trait Mapping Genetics) 2020-04-20 22:19:19 Poster https://tagc2020.figshare.com/articles/poster/Characterization_of_a_potential_gene_interaction_between_spr-5_met-2_and_mep-1_in_determining_germline_versus_soma_in_C_elegans/12149796 <p>In <i>C. elegan</i>s, epigenetic modifier proteins aid in the activation and repression of gene expression that is required to distinguish germline from soma. Here we explore the possibility that the early actions of SPR-5 and MET-2 are reinforced in the embryo (until a germline is formed) by coordinating with other transcriptional regulators such as MEP-1. Our data show, a developmental delay in spr-5; met-2; mep-1 mutants is more severe than in spr-5; met-2 mutants. We show, gene misexpression in spr-5; met-2; mep-1 mutants is further amplified when compared to spr-5; met-2 mutants at the same gene loci. Moreover, spr-5; met-2; mep-1 mutants exhibit differentially expressed genes specifically enriched in germline targets. Collectively our data support a conclusion that MEP-1 collaborates with SPR-5 and MET-2 to suppress inappropriate germline gene expression in the soma.</p><br>