10.6084/m9.figshare.12149796.v1
Karen Schmeichel
Karen
Schmeichel
Onur Birol
Onur
Birol
David Katz
David
Katz
Sindy Chavez
Sindy
Chavez
Brandon Carpenter
Brandon
Carpenter
Jovan Brockett
Jovan
Brockett
Characterization of a potential gene interaction between spr-5, met-2, and mep-1 in determining germline versus soma in C. elegans
TAGC 2020
2020
Caenorhabditis elegans
bioinformatics analyses
Epigenetic Modifications
Development
cell biology experiments
RNAi knockdown mutants
Differentially expressed gene (DEG)
transcriptomics data
Bioinformatics
Cell Biology
Epigenetics (incl. Genome Methylation and Epigenomics)
Genetics
Gene Expression (incl. Microarray and other genome-wide approaches)
Genomics
Molecular Biology
Quantitative Genetics (incl. Disease and Trait Mapping Genetics)
2020-04-20 22:19:19
Poster
https://tagc2020.figshare.com/articles/poster/Characterization_of_a_potential_gene_interaction_between_spr-5_met-2_and_mep-1_in_determining_germline_versus_soma_in_C_elegans/12149796
<p>In <i>C. elegan</i>s, epigenetic modifier proteins aid in the activation and repression of
gene expression that is required to distinguish germline from soma. Here we explore the
possibility that the early actions of SPR-5 and MET-2 are reinforced in the
embryo (until a germline is formed) by coordinating with other transcriptional
regulators such as MEP-1. Our data show, a developmental delay in spr-5; met-2; mep-1 mutants is more severe than in
spr-5; met-2 mutants. We show, gene misexpression in spr-5; met-2; mep-1 mutants is further amplified when compared to spr-5; met-2 mutants
at the same gene loci. Moreover, spr-5; met-2; mep-1 mutants exhibit differentially expressed
genes specifically enriched in germline targets. Collectively our data support
a conclusion that MEP-1 collaborates with SPR-5 and MET-2
to suppress inappropriate germline gene expression in the soma.</p><br>