DNA methylation is required for expression of regional gene expression programs in the zebrafish intestine Gilberto Padilla Mercado Colin R. Lickwar Jia Wen Lindsey Marjoram Michel Bagnat John F. Rawls 10.6084/m9.figshare.12149409.v1 https://tagc2020.figshare.com/articles/poster/DNA_methylation_is_required_for_expression_of_regional_gene_expression_programs_in_the_zebrafish_intestine/12149409 Describes findings that DNA methylation is important for small intestinal signature gene expression programs. Uses zebrafish transgenics (4.5fabp2:DsRed, NFkB:GFP, cldn15BAC:GFP, tnfa:GFP). Includes exploratory data analysis of RNA-Seq and single-cell RNA seq data, the latter analyzed with the package Seurat. Live stereoscopic fluorescence imaging quantified with FIJI image processing software. 2020-04-20 22:40:13 DNA methylation zebrafish intestine gene regulation epigenetics DNA methyltransferases 1 fatty acid binding protein transgenics Single-cell RNA-Seq analysis RNA-Seq Animal Cell and Molecular Biology Epigenetics (incl. Genome Methylation and Epigenomics)