A Role for UNC-45 in Maintaining Myosin During Aging
Courtney Christian
Hiroshi Qadota
Guy M. Benian
10.6084/m9.figshare.12148533.v1
https://tagc2020.figshare.com/articles/poster/A_Role_for_UNC-45_in_Maintaining_Myosin_During_Aging/12148533
Using immunostaining with antibodies to myosin
heavy chain A (MHC A), we show that there is a gradual decline in motility
beginning at day 4 and the number of A-bands (a measure of thick filament
assembly) beginning at day 8 adults. By day 12 and especially day 16, there is
also disorganization of A-bands. This disorganization appears similar
to that
of unc-45 ts
mutants grown at the restrictive temperature. We have found that in C.
elegans a
decline in hsp-90 mRNA
(day 2) directly precedes a decline in HSP-90 protein (day3), which directly
precedes a decline in UNC-45 protein (day4), which then in turn precedes a
decline in Myosin B (day8), the main client of UNC-45. unc-45,
unc-54, and myo-3 mRNA
decline immediately after reaching maturity (day 1) and remain stable, though
low – which is expected for proteins assembled into the sarcomere. We also
observe a decrease in UNC-45 protein, but not mRNA, in an hsp-90 loss
of function mutant, suggesting a role for HSP-90 in UNC-45 regulation and/or
protein stabilization. We also observe early onset of sarcopenia when UNC-45 is
lost during adulthood and an increase in an unknown UNC-45 post translational
modification with age.
2020-04-20 23:03:51
aging-related
C. elegans
muscle
Sarcopenia
UNC-45
Aged Health Care