10.6084/m9.figshare.12141993.v1
J. Dylan Shropshire
J.
Dylan Shropshire
Mahip Kalra
Mahip
Kalra
Seth Bordenstein
Seth
Bordenstein
Characterizing bacteriophage proteins that hijack arthropod reproduction
TAGC 2020
2020
Drosophila melanogaster
Wolbachia
Phage WO
Cytoplasmic incompatibility
Reproductive manipulation
cifA
cifB
Microbiology
Quantitative Genetics (incl. Disease and Trait Mapping Genetics)
Genetics
Microbial Genetics
Developmental Genetics (incl. Sex Determination)
2020-04-20 23:36:43
Poster
https://tagc2020.figshare.com/articles/poster/Characterizing_bacteriophage_proteins_that_hijack_arthropod_reproduction/12141993
Wolbachia are maternally-inherited symbionts that cause cytoplasmic incompatibility (CI). Dual expression of the two phage WO genes cifA and cifB in males causes CI, while expression of cifA in females rescues CI. Structural homology-based analyses suggest that CifA and CifB proteins have three putative functional domains each, but the relative importance of conserved sites in these regions to CI and rescue is unknown. Here, we use site-directed substitution mutagenesis and transgenic expression in uninfected Drosophila melanogaster to determine the functional importance of conserved amino acids in CifA and CifB proteins.<br>