Bondy-Chorney, Emma Abramchuk, Iryna Nasser, Rawan Holinier, Charlotte Denoncourt, Alix Baijal, Kanchi Mccarthy, Liam Khacho, Mireille Lavallee-Adam, Mathieu Downey, Michael A broad response to intracellular long-chain polyphosphate in human cells Polyphosphates (polyP) are ancient molecules comprised of inorganic phosphates joined by high energy phosphoanhydride bonds in chains of 3-1000 units in length. In this study, we describe a system to produce polyP in mammalian cells by ectopic expression of the E. coli ppk1+ gene. Production of the EcPpk1 protein results in polyP accumulation throughout the cell, including the nucleus. Using transcriptomic and proteomic analyses, we demonstrate a broad impact of polyP on diverse pathways, including activation of the ERK1/2-ERG1 signaling axis. PolyP accumulation also results in redistribution of several chromatin bound proteins and a translation initiation factor from the nucleus to the cytoplasm. Our work will serve as a novel resource to interrogate polyP biology in higher eukaryotes. <br> Polyphosphate;lysine polyphosphorylation;ERK1/2-ERG1 signaling axis;protein redistribution;Animal Cell and Molecular Biology;Bioinformatics;Cell Biology;Enzymes;Gene Expression (incl. Microarray and other genome-wide approaches);Genomics;Molecular Biology 2020-04-20
    https://tagc2020.figshare.com/articles/poster/A_broad_response_to_intracellular_long-chain_polyphosphate_in_human_cells/12140991
10.6084/m9.figshare.12140991.v1