Glycosylation of specific Notch EGF repeats by Ofut1 and Fringe regulates Notch signaling in Drosophila
Ashutosh Pandey
Beth M. Harvey
Mario F Lopez
Atsuko Ito
Robert S. Haltiwanger
Hamed Jafar-Nejad
10.6084/m9.figshare.12127095.v1
https://tagc2020.figshare.com/articles/poster/Glycosylation_of_specific_Notch_EGF_repeats_by_Ofut1_and_Fringe_regulates_Notch_signaling_in_Drosophila/12127095
We have reported important roles in Drosophila Notch signaling for GlcNAc-fucose-O glycans on three sites: EGF8, 9 and 12. O-fucose monosaccharide on EGF12 (in the absence of Fringe) is essential for Delta-mediated lateral inhibition in embryos. However, wing vein development depends on addition of GlcNAc to EGF8 and 12 by Fringe, with minor contribution from EGF9. Fringe modifications of EGF8 and 12 together prevent Notch from cis-inhibiting Serrate, thereby promoting normal wing margin formation. Our work shows the combinatorial and context-dependent roles of GlcNAc-fucose-O glycans on these sites in Drosophila Notch-ligand interactions.
2020-04-20 23:51:38
Drosophila
Notch signaling
Fringe
O-glycosylation
O-Fut1
Genetics
Molecular Biology
Developmental Biology